consequently, they must be substantively and formally established by law, 425 pursue a legitimate aim, and comply with the requirements of suitability, necessity and proportionality. 426 In the instant case, the Court has underlined that the “absolute right to life of the embryo” as grounds for the restriction of the rights involved, is not supported by the American Convention (supra para. 264); thus, it is not necessary to make a detailed analysis of each of these requirements, or to assess the disputes regarding the declaration of unconstitutionality in the formal sense based on the presumed violation of the principle of legal reserve. Despite the foregoing, the Court considers it appropriate to indicate the way in which the sacrifice of the rights involved in this case was excessive in comparison to the benefits referred to with the protection of the embryo 427. 274. To this end, the restriction would have to protect prenatal life significantly, without annulling the rights to private life and to found a family. In order to weigh these factors the Court must analyze: (i) the level of harm to one of the rights at stake, determining whether the level of this harm was serious, intermediate or moderate; (ii) the importance of ensuring the contrary right, and (iii) whether ensuring the latter justifies restricting the former. 428 275. The European Court of Human Rights has indicated that the possible conflict between the right to private life, which includes the rights to autonomy and to the free development of the persona, and “the possibility that, in certain circumstances, safeguards may be extended to the unborn child [must be] determined by weighing various […] rights or freedoms claimed by a mother and a father involved in a relationship with one another or vis-à-vis the foetus.” 429 “This Court has stated that “undue deference for the protection of prenatal life or on the basis that the right of the future mother to respect for her private life is of a lower rank, does not constitute a reasonable and proportionate weighing up of competing rights and interests.” 430 Also, in the case of Costa and Pavan v. Italy, the European Court considered that the absolute prohibition of pre-implantation diagnosis was not proportionate, owing to the inconsistent domestic legislation concerning reproductive rights that, while prohibiting the pre-implantation diagnosis, permitted the termination of the pregnancy if the fetus subsequently revealed symptoms of a grave illness detectable by preimplantation diagnosis. 431 276. The Court will weigh up the factors analyzing: (i) the severity of the interference that took place in the rights to private and family life and the other rights involved in the instant case. In addition, this severity is analyzed based on the disproportionate impact in relation to: (ii) disability, (iii) gender, and (iv) socio-economic situation. Lastly, the Court will analyze (v) the dispute on the alleged embryonic loss. D.1) Severity of the limitation of the rights involved in this case 425 Cf. The Word “Laws” in Article 30 of the American Convention on Human Rights. Advisory Opinion OC-6/86 of May 9, 1986. Series A No. 6, paras. 35 and 37. 426 Cf. Case of Tristán Donoso v. Panama. Preliminary objection, merits, reparations and costs. Judgment of January 27, 2009. Series C No.193, para. 56, and Case of Atala Riffo and daughters v. Chile, para. 164. 427 Case of Kimel v. Argentina, para. 83, and Case of Chaparro Álvarez and Lapo Íñiguez, para. 93. 428 Case of Kimel v. Argentina, para. 84. 429 Cf. ECHR, Case of Vo. v. France (No. 53924/00), Judgment of 8 July 2004, para. 80, Case of RR v. Poland (No. 27617/04), Judgment of 26 May 2011, para. 181 430 Cf. ECHR, Case of A, B and C v. Ireland (No. 25579/05), Grand Chamber. Judgment of 16 December 2010, para. 238. 431 Cf. ECHR, Case of Costa and Pavan v. Italy, (No. 54270/10), Judgment of 28 August 2012, para. 71.